Microsatellite Primers for Two Threatened Orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)

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Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).

PREMISE OF THE STUDY The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami's urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae). METHODS AND RESULTS Ten microsatellites were developed for each species. For E. tampensis sampled ...

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A new set of microsatellite primers for Coelogyne fimbriata (Orchidaceae) and cross-amplification in C. ovalis1

PREMISE OF THE STUDY Declining orchid populations have made it necessary to prioritize the study of population structure and genetic diversity for species including Coelogyne fimbriata (Orchidaceae). METHODS AND RESULTS A biotin-streptavidin capture method was used to construct a microsatellite library for C. fimbriata. A total of 15 polymorphic nuclear microsatellite loci were isolated and c...

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Encyclia inopinata (Orchidaceae, Laeliinae) a new species from Mexico

A new species of Encyclia from Mexico, Encyclia inopinata, is described and illustrated. This species is similar to Encyclia diota but it can be distinguished by its usually more robust plants with 2-3 leaves per pseudobulb and its flowers with longer and narrower sepals (1.8±0.1 × 0.63±0.03 cm in Encyclia inopinata versus 1.48 ±0.14 × 0.65±0.06 cm in Encyclia diota) and petals (1.7±0.05 × 0.59...

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Stable Epigenetic Effects Impact Adaptation in Allopolyploid Orchids (Dactylorhiza: Orchidaceae)

Epigenetic information includes heritable signals that modulate gene expression but are not encoded in the primary nucleotide sequence. We have studied natural epigenetic variation in three allotetraploid sibling orchid species (Dactylorhiza majalis s.str, D. traunsteineri s.l., and D. ebudensis) that differ radically in geography/ecology. The epigenetic variation released by genome doubling ha...

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Characterization of microsatellite primers in the endangered orchid Phaius australis and cross-amplification to P. bernaysii (Orchidaceae)1

Premise of the study The swamp orchid, Phaius australis (Orchidaceae), is nationally endangered due to illegal collection and habitat loss and fragmentation, resulting in a disjunct distribution in spring and coastal wetland ecotones along Australia's east coast. Polymorphic microsatellite markers were developed to study genetic diversity and population structure for conservation and restoratio...

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ژورنال

عنوان ژورنال: Applications in Plant Sciences

سال: 2016

ISSN: 2168-0450

DOI: 10.3732/apps.1500095